Journal: Stem Cell Research & Therapy
Article Title: Methylsulfonylmethane enhances MSC chondrogenic commitment and promotes pre-osteoblasts formation
doi: 10.1186/s13287-021-02396-5
Figure Lengend Snippet: Effects of MSM in the osteogenic maturation. RUNX2 gene expression was reduced while the expression of SPARC and SPP1 ( A ), as well the number of osteocalcin positive cells ( B ) increased in MSM-treated cells after 14 days of osteogenic differentiation. After 21 days of differentiation calcium deposition, evaluated by alizarin red staining (ARS), increased in cells treated with MSM ( C ). * p <0.05; ** p <0.01
Article Snippet: Briefly, predesigned, gene-specific primers, and probe sets for each gene (RUNX2, hs00231692_m1; OSTERIX (SP7), hs00541729_m1; COLLAGEN, TYPE I, ALPHA 2 (COL1A2), hs01028956_m1; OSTEONECTIN (SPARC), hs00234160_m1; OSTEOPONTIN (SPP1), hs00167093_m1, SOX9, hs01107818_m1; COMP, hs004359-m1; COL2A1, hs00264051_m1; B2M, hs999999_m1 (housekeeping); GAPDH, 0802021 (housekeeping); sox9a, Dr03112282_m1; col2a1a, Dr03099270_m1; miR-146b-5p, 474220; RNU44, 001094) were obtained from assay-on-demand gene expression products (Thermo Fisher Corporation, Waltham, MA, USA).
Techniques: Gene Expression, Expressing, Staining